Application
ChIP
Sample
Rat Cell lysate - whole cell (Rat normal liver cells fresh isolated)
Specification
Rat normal liver cells fresh isolated
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Detection step
Real-time PCR
Negative control
Normal IgG
Other product details
Dilution
1/100
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 25°C · Diluent: Antibody buffer from Sigma Imprint ChIP
Additional data
Additional Notes
Sigma Imprint ChIP Kit is used. Follow its protocol. 1ug antibody was used in a reaction well provided by Imprint ChIP kit.
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提交于 Sep 25 2008