Application
Western blot
Sample
Human Cell lysate - whole cell (DLD1)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
DLD1
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 25°C
Dilution
1/5000
Detection
Detection method
ECL Prime
Exposure
50 second(s)
Bands
Specific: 15 kDa Non-specific: 12 kDa
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提交于 Feb 13 2017