Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - nuclear (U2OS)
Specification
U2OS
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk in TBST
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Goat Anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Dilution
1/1000
Detection
Detection method
Fluorescence
Bands
Specific: 17 kDa
Exposure
5 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 May 15 2014