Application
Flow Cytometry
Sample
Zebrafish Cell (Dissociated cells from zebrafish testicle)
Permeabilization
Yes - Methanol 90%
Gating Strategy
Cell populations were gated acording to their DNA content.
Specification
Dissociated cells from zebrafish testicle
Preparation
Cell harvesting/tissue preparation method: Cells dissociated with collagenase and fixed with paraformaldehylde
Sample buffer: PBS
Sample buffer: PBS
Fixation
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA + 10% NGS in PBS
Dilution
1/200
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Secondary Antibody, Ale
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 488
Additional data
Additional Notes
I include an image in which it can be see the flow cytometry plot example as well as an example of the fluorescence intensity for each cell population.
A representative graph is also included, to see the ratio of fluorescence using different dilutions of primary antibody (the respective negative control without the first antibody is added.
PubMed ID
GR232399: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Aug 01 2017