Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Methanol
Other product details
Dilution
1/200
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Mouse Cy3
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/200
Additional data
Additional Notes
Asynchronous HeLa cells were either methanol or paraformaldehyde fixed, immunofluorescently labeled and counterstained with DAPI. This antibody appears to recognize a nuclear epitope that is likely to be H2B, but it also recognizes an eptiope localizing to the nucleoli of interphase cells. In PF fixed samples, it weakly reacts during interphase and does not appear to recognize anything during mitosis.
Abcam response
Thank you for your valuable feedback. ICC/IF was not a tested application for ab52484 and it seems that this antibody does not recognize Histone H2B under these experimental conditions. Thank you for your time - we hope that this information will be helpful for future customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
提交于 May 09 2008