Application
Western blot
Sample
Cow Recombinant protein (Calf)
Loading amount
0.25 µg
Specification
Calf
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/20000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TBS+0.1% Tween 20
Secondary antibody
Name
Non-Abcam antibody was used: Biorad
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL plus
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 10 kDa Non-specific: 6 kDa
Positive control
recombinant H2B
Negative control
recombinant H2A
Additional data
Additional Notes
Haven't tested with cell lysate yet. But it is very specific based on western using recombinant protein.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Dec 05 2007