Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H2A.Z antibody [4A4]

Poor
Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% TritonX100 in PBS

Other product details

Dilution
1/100
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-Rat Cy3
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/200

Additional data

Additional Notes
Asynchronous HeLa were paraformaldehyde fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. No nuclear staining signal was apparent indicating that the antibody does not appear to recognize its epitope under these conditions in these cells. Methanol-fixed samples were also tried and did not produce a nuclear signal.
Abcam response
Thank you for your feedback. We have also found that this antibody does not seem to be suitable for use in ICC/IF experiments (see application notes section). We hope this information might be useful for future users.
Username

Dr. Kirk Mcmanus

Verified customer

提交于 Mar 29 2010

注册