Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H2A.Z antibody [4A4]

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Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa)
Specification HeLa
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% TritonX100 in PBS

Other product details

Dilution 1/100
Incubation time 16 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS

Secondary antibody

Name Non-Abcam antibody was used: Goat anti-Rat Cy3
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution 1/200

Additional data

Additional Notes Asynchronous HeLa were paraformaldehyde fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. No nuclear staining signal was apparent indicating that the antibody does not appear to recognize its epitope under these conditions in these cells. Methanol-fixed samples were also tried and did not produce a nuclear signal.
Abcam response Thank you for your feedback. We have also found that this antibody does not seem to be suitable for use in ICC/IF experiments (see application notes section). We hope this information might be useful for future users.
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Dr. Kirk Mcmanus

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提交于 Mar 29 2010