Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Histone H2A.Z antibody [4A4]

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Abreviews
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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Pancreatic cancer)
Specification Pancreatic cancer
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization No
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C

Other product details

Dilution 1/1000
Incubation time 45 minute(s) · Temperature: 20°C · Diluent: Antibody Diluent (Dako)

Secondary antibody

Name Non-Abcam antibody was used: Anti-rabbit/mouse HRP polymer (pre-diluted, Dako)
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer

Additional data

Additional Notes After the primary antibody incubation a rabbit anti-rat link (Dako) was used (1/400 for 30min at 20oC) before the anti-rabbit polymer HRP was applied.
Three H2A.Z antibodies were tested in parallel (AB80150, AB80153 and AB4462). The staining pattern was very similar in each case and all three could probably be used up to 1/5000 dilution in order to reduce background. Other possible suggestions would be a longer antigen retrieval and an overnight primary incubation at 4oC.
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提交于 Mar 11 2010