Anti-Histone H2A.Z抗体[4A4] (ab80150)

概述

  • 产品名称
    Anti-Histone H2A.Z抗体[4A4]
    参阅全部 Histone H2A.Z 一抗
  • 描述
    大鼠单克隆抗体[4A4] to Histone H2A.Z
  • 经测试应用
    适用于: IHC-P, WB, Flow Cytmore details
    不适用于: ICC/IF
  • 种属反应性
    与反应: Human, Recombinant fragment
    预测可用于: Mouse, Rat, Cow, Pig, Xenopus laevis, Zebrafish, Orangutan
  • 免疫原

    Synthetic peptide corresponding to Human Histone H2A.Z.

  • 阳性对照
    • This antibody gave a positive signal when used to probe Histone H2A.Z recombinant protein.
  • 常规说明

    This antibody clone is manufactured by Abcam.

性能

  • 形式
    Liquid
  • 存放说明
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液
    Preservative: 0.02% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • 纯度
    IgG fraction
  • 克隆
    单克隆
  • 克隆编号
    4A4
  • 同种型
    IgG2b
  • 研究领域

应用

Our Abpromise guarantee covers the use of ab80150 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 13 kDa).
Flow Cyt Use 1µg for 106 cells. ab18536-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
  • 应用说明
    Is unsuitable for ICC/IF.
  • 靶标

    • 功能
      Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.
    • 序列相似性
      Belongs to the histone H2A family.
    • 翻译后修饰
      Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
      Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.
      Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.
      Not phosphorylated.
    • 细胞定位
      Nucleus. Chromosome.
    • Information by UniProt
    • 数据库链接
    • 别名
      • H2A histone family member Z antibody
      • H2A.z antibody
      • H2A/z antibody
      • H2afz antibody
      • H2AZ antibody
      • H2AZ_HUMAN antibody
      • Histone H2A.Z antibody
      • MGC117173 antibody
      see all

    图片

    • All lanes : Anti-Histone H2A.Z antibody [4A4] (ab80150) at 1 µg/ml

      Lane 1 : Histone H2A.Z recombinant protein
      Lane 2 : Histone H2A recombinant protein

      Lysates/proteins at 0.5 µg per lane.

      Secondary
      Goat polyclonal to Rat IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution

      Predicted band size : 13 kDa
      Observed band size : 17 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 30 kDa. We are unsure as to the identity of these extra bands.
    • ab80150 (1/1000) staining Histone H2A.Z in paraffin-embedded Human pancreatic cancer tissue.  Tissue underwent fixation in formaldehyde, peroxidase blocking, protein blocking and heat mediated antigen retrieval.  After the primary antibody incubation a rabbit anti-rat link was used before the application of the secondary antibody which was goat anti rabbit/mouse conjugated to HRP.  For further experimental details please refer to abreview.

      See Abreview

    • Overlay histogram showing HeLa cells stained with ab80150 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80150, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2b [RTK4530] ab18541, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    文献

    ab80150 has not yet been referenced specifically in any publications.

    客户评价及客户问答

    Thank you for contacting me.

    I am sorry that the Anti-Histone H2A.Z antibody (ab97966) has now been removed from the catalogue. I am sorry but I was not aware of this change when I suggested you test it. Let me reassure you that the antibody ...

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    Thank you for contacting Abcam.

    All of our products are for research purposes only and are not certified for clinical research applications.

    I am sorry that I could not be more helpful in this matter.

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% TritonX100 in PBS
    Username

    Dr. Kirk Mcmanus

    Verified customer

    提交于 Mar 29 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Pancreatic cancer)
    Specification
    Pancreatic cancer
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
    Username

    Mr. Antibody Solutions

    Verified customer

    提交于 Mar 11 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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