概述
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产品名称Anti-Histone H2A.Z抗体[4A4]
参阅全部 Histone H2A.Z 一抗 -
描述大鼠单克隆抗体[4A4] to Histone H2A.Z
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宿主Rat
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经测试应用适用于: IHC-P, WB, Flow Cytmore details
不适用于: ICC/IF -
种属反应性与反应: Human, Recombinant fragment
预测可用于: Mouse, Rat, Cow, Pig, Xenopus laevis, Zebrafish, Orangutan
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免疫原
Synthetic peptide corresponding to Human Histone H2A.Z.
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阳性对照
- This antibody gave a positive signal when used to probe Histone H2A.Z recombinant protein.
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常规说明
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... -
纯度IgG fraction
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克隆单克隆
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克隆编号4A4
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同种型IgG2b
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研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab80150 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 13 kDa). | |
| Flow Cyt | Use 1µg for 106 cells. ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
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靶标
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功能Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division.
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序列相似性Belongs to the histone H2A family.
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翻译后修饰Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.
Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.
Not phosphorylated. -
细胞定位Nucleus. Chromosome.
- Information by UniProt
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数据库链接
- Entrez Gene: 287016 Cow
- Entrez Gene: 3015 Human
- Entrez Gene: 51788 Mouse
- Entrez Gene: 58940 Rat
- Omim: 142763 Human
- SwissProt: P0C0S4 Cow
- SwissProt: P0C0S5 Human
- SwissProt: P0C0S6 Mouse
see all -
别名
- H2A histone family member Z antibody
- H2A.z antibody
- H2A/z antibody
see all
图片
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![Western blot - Histone H2A.Z antibody [4A4] (ab80150)](http://www.abcam.cn/ps/datasheet/images/80/ab80150/Histone-H2AZ-Primary-antibodies-ab80150-1.jpg)
Western blot - Histone H2A.Z antibody [4A4] (ab80150)All lanes : Anti-Histone H2A.Z antibody [4A4] (ab80150) at 1 µg/ml
Lane 1 : Histone H2A.Z recombinant protein
Lane 2 : Histone H2A recombinant protein
Lysates/proteins at 0.5 µg per lane.
Secondary
All lanes : Goat polyclonal to Rat IgG - H&L - Pre-Adsorbed (HRP) at 1/10000 dilution
Predicted band size: 13 kDa
Observed band size: 17 kDa (why is the actual band size different from the predicted?)
Additional bands at: 30 kDa. We are unsure as to the identity of these extra bands. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H2A.Z antibody [4A4] (ab80150)](http://www.abcam.cn/ps/datasheet/images/80/ab80150/Histone-H2AZ-Primary-antibodies-ab80150-8.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Histone H2A.Z antibody [4A4] (ab80150)This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.ab80150 (1/1000) staining Histone H2A.Z in paraffin-embedded Human pancreatic cancer tissue. Tissue underwent fixation in formaldehyde, peroxidase blocking, protein blocking and heat mediated antigen retrieval. After the primary antibody incubation a rabbit anti-rat link was used before the application of the secondary antibody which was goat anti rabbit/mouse conjugated to HRP. For further experimental details please refer to abreview.
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![Flow Cytometry - Anti-Histone H2A.Z antibody [4A4] (ab80150)](http://www.abcam.cn/ps/datasheet/images/80/ab80150/Histone-H2AZ-Primary-antibodies-ab80150-9.jpg)
Flow Cytometry - Anti-Histone H2A.Z antibody [4A4] (ab80150)Overlay histogram showing HeLa cells stained with ab80150 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80150, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rat IgG2b [RTK4530] ab18541, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
文献
ab80150 has not yet been referenced specifically in any publications.
客户评价及客户问答
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