Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Na-citrate pH6
Sample
Mouse Tissue sections (Liver, P5)
Specification
Liver, P5
Permeabilization
Yes - Triton 0,05%
Fixative
Paraformaldehyde
Other product details
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS/Triton 0,05%
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: ALEXAFluor™ 555 Donkey ANTI RABBIT
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 555
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 555
Dilution
1/250
Additional data
Additional Notes
Slides were rehydrated and then boiled for antigen retrieval in Citrate pH 6 buffer for 15min in standard microwave oven at 650W. Nuclei were stained with Hoechst.
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提交于 Oct 07 2014