Application
Western blot
Loading amount
0.5 µg
Gel Running Conditions
Reduced Denaturing (Criterion TGX 4-20% (BioRad))
Sample
Human Purified protein (Recombinant octamers & purified K562 cell histones)
Specification
Recombinant octamers & purified K562 cell histones
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Donkey-anti-rabbit-HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL Super Signal West Dura
Bands
Specific: 15 kDa
Exposure
5 minute(s) and 0 second(s)
Negative control
Recombinant octamers contiaining H2A.Z.1 and H2A.Z.2.1.
Positive control
Predicted size 14 kDa.
Observed size 15 kDa.
Additional data
Additional Notes
Lane 1 Native recombinant octamers K562 cells (0.5 micrograms).
Lane 2 Recombinant Human octamers containing H2A (0.5 micrograms).
Lane 3 Recombinant Human octamers containing H2A.Z.2.1 (0.5 micrograms).
Lane 4 Recombinant Human octamers containing H2A.Z.1 (0.5 micrograms).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Ragnhild Eskeland
Verified customer
提交于 Jan 27 2015