Application
Western blot
Sample
Human Cell lysate - whole cell (MRC5VA Lung Fibroblasts)
Loading amount
15 µg
Specification
MRC5VA Lung Fibroblasts
Gel Running Conditions
Reduced Denaturing (9%)
Blocking step
Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Concentration
0.5 µg/ml
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: PBS/Tween
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Rabbit HRP (Dako)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: ~30kDa kDa
Positive control
Just MRC5VA
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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提交于 Feb 10 2011