Anti-Hex抗体(ab34222)
Key features and details
- Rabbit polyclonal to Hex
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-Hex抗体
参阅全部 Hex 一抗 -
描述
兔多克隆抗体to Hex -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse
预测可用于: Rat, Human, Pig -
免疫原
Synthetic peptide corresponding to Human Hex aa 200 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab28465) -
阳性对照
- This antibody gave a positive signal in Mouse Liver Tissue Lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab34222于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 30 kDa).
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 30 kDa). |
靶标
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功能
Recognizes the DNA sequence 5'-ATTAA-3'. Transcriptional repressor. May play a role in hematopoietic differentiation. Establishes anterior identity at two levels; acts early to enhance canonical WNT-signaling by repressing expression of TLE4, and acts later to inhibit NODAL-signaling by directly targeting NODAL. -
组织特异性
Liver and promyelocytic leukemia cell line HL60. -
序列相似性
Contains 1 homeobox DNA-binding domain. -
发展阶段
Expressed during hematopoiesis. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 3087 Human
- Entrez Gene: 15242 Mouse
- Entrez Gene: 397232 Pig
- Entrez Gene: 79237 Rat
- Omim: 604420 Human
- SwissProt: Q03014 Human
- SwissProt: P43120 Mouse
- Unigene: 118651 Human
see all -
别名
- Hematopoietically expressed homeobox antibody
- Hematopoietically-expressed homeobox protein HHEX antibody
- HEX antibody
see all
图片
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Anti-Hex antibody (ab34222) at 1 µg/ml + Liver (Mouse) Tissue Lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 30 kDa
Observed band size: 37 kDa why is the actual band size different from the predicted?
Additional bands at: 42 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab34222 被引用在 5 文献中.
- Watanabe H et al. Transcription Factor Hematopoietically Expressed Homeobox Protein (Hhex) Negatively Regulates Osteoclast Differentiation by Controlling Cyclin-Dependent Kinase Inhibitors. JBMR Plus 6:e10608 (2022). PubMed: 35434453
- Guo Y et al. CK2-induced cooperation of HHEX with the YAP-TEAD4 complex promotes colorectal tumorigenesis. Nat Commun 13:4995 (2022). PubMed: 36008411
- Dyikanov DT et al. Optimization of CRISPR/Cas9 Technology to Knock Out Genes of Interest in Aneuploid Cell Lines. Tissue Eng Part C Methods 25:168-175 (2019). PubMed: 30747044
- Wadey KS et al. Protein kinase CK2 inhibition suppresses neointima formation via a proline-rich homeodomain-dependent mechanism. Vascul Pharmacol 99:34-44 (2017). WB, IHC-P . PubMed: 28927755
- Stepanova V et al. Urokinase-type Plasminogen Activator (uPA) Promotes Angiogenesis by Attenuating Proline-rich Homeodomain Protein (PRH) Transcription Factor Activity and De-repressing Vascular Endothelial Growth Factor (VEGF) Receptor Expression. J Biol Chem 291:15029-45 (2016). Mouse . PubMed: 27151212