Application
Western blot
Sample
Human Cell lysate - whole cell (transfected Huh7)
Specification
transfected Huh7
Blocking step
BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: anti-mouse immunoglobulins
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 42, 39, 36, 33 kDa
Positive control
Cell lysate from transfected Huh7 and cos cells. PEG precipitated as well as sucrose cushioned supernatant from transfected cells.
Negative control
Untransfected cells. Low level background is observed, but background bands are usually higher than the L&M bands.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Miss. Nadia Warner
Verified customer
提交于 Feb 07 2006