Application
Western blot
Sample
Mouse Tissue lysate - whole (lung supernatant)
Gel Running Conditions
Reduced Denaturing (12,5%)
Loading amount
85 µg
Treatment
hyperoxia for 18 hrs
Specification
lung supernatant
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBR + Tween (0.1%)
Dilution
1/500
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Amersham anti-rabbit IgG, horseradish peroxidase-
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
chemiluminescence
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 36 kDa
Positive control
brain
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Sandra Sobocanec
Verified customer
提交于 May 17 2011