Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Permeabilization
Yes - 0.5% Triton
Specification
HeLa
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5% serum
Dilution
1/1000
Secondary antibody
Dilution
1/2500
Name
Non-Abcam antibody was used: Donkey anti Rabbit 555
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 555
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 555
Additional data
Additional Notes
Dox inducible HA-H2A expressing HeLa treated with Dox for 24 hours to induce expression.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Feb 01 2017