HeLa全细胞裂解物(ab150035)
概述
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产品名称
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常规说明
We recommend aliquoting the extracts into single use fractions and then storing them at -80°C.
This HeLa whole cell was prepared using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT).
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经测试应用
适用于: WBmore details
性能
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Mycoplasma free
Yes -
形式
Liquid -
存放说明
Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.50
Constituent: 10% (R*,R*)-1,4-Dimercaptobutan-2,3-diol -
Concentration information loading...
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裂解物说明
This HeLa whole cell was prepared using a standard whole cell lysate protocol.
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab150035于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent concentration.
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说明 |
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WB
Use at an assay dependent concentration. |
图片
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All lanes : Anti-Actin antibody - Loading Control (ab1801) at 1 µg/ml
Lane 1 :HeLa whole cell lysate (ab150035)
Lane 2 :HeLa nuclear extract lysate (ab150036)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Observed band size: 42 kDa why is the actual band size different from the predicted? -
All lanes : Anti-TATA binding protein TBP antibody - Nuclear Loading Control and ChIP Grade (ab63766) at 1 µg/ml
Lane 1 :HeLa whole cell lysate (ab150035)
Lane 2 :HeLa nuclear extract lysate (ab150036)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Observed band size: 45 kDa why is the actual band size different from the predicted? -
All lanes stained with Optiblot Blue (ab119211) (20 ml)
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab150035)
Lysates/proteins at 10 µg per lane. -
All lanes : Anti-beta Tubulin antibody (ab21057) at 1 µg/ml
Lane 1 :HeLa whole cell lysate (ab150035)
Lane 2 :HeLa nuclear extract lysate (ab150036)
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
Observed band size: 51 kDa why is the actual band size different from the predicted? -
All lanes : Anti-HDAC1 antibody (ab19845) at 1 µg/ml
Lane 1 :HeLa whole cell lysate (ab150035)
Lane 2 :HeLa nuclear extract lysate (ab150036)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Observed band size: 60 kDa why is the actual band size different from the predicted?
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab150035 尚未被引用在任何文献中。