HRP Stability Conjugate试剂(ab171537)
概述
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产品名称
HRP Stability Conjugate试剂
参阅全部 ELISA buffer 试剂 -
经测试应用
适用于: ELISAmore details
不适用于: IHC-Fr or IHC-P -
常规说明
Abcam’s HRP Stability Conjugate Reagent (ab171537) was developed specifically for Horseradish Peroxidase (HRP) conjugates in solution. ab171537 is readily incorporated into virtually any assay production protocol. Conjugates can be added directly to ab171537 and are then ready for storage and use. It is also an excellent diluent, and often decreases background and increases signal for improved signal-to-noise ratios. ab171537 prevents the loss of catalytic activity while it maintains the conformation of the antibody or protein-antigen portion of the conjugate.
98% Proprietary component
Recommendations for Use of ab171537 HRP Stability Conjugate Reagent
- For optimal stability and performance, use ab171537 at 100% concentration
- Dilute your HRP conjugate to optimum working concentration in the HRP Stability Conjugate Reagent solution
- Use diluted conjugate working solution in your assay as normal
- Or, preferably, store away from direct light at room temperature and use as a stock solution for optimum assay-to-assay consistency
NOTE – When storing any conjugate/ HRP Stability Conjugate Reagent solution for an extended period of time: If a phosphate buffer is used for dilution we recommend a final concentration of no more than 5 mM buffer to avoid the possibility of initiating precipitation.
性能
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形式
Liquid -
存放说明
Store at room temperature. -
存储溶液
pH: 6.20
Preservative: 0.02% 5-Bromo-5-nitro-1,3-dioxane
Constituents: 0.02% 2-methyl-4-isothiazolin-3-one, 0.0024% StabilZyme® HRP Conjugate Stabilizer
Further information:
Complete pH range: 6.2 - 6.7
98% Proprietary component
Contains BSA -
Concentration information loading...
相关产品
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab171537于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ELISA |
Use at an assay dependent concentration.
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说明 |
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ELISA
Use at an assay dependent concentration. |
图片
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HRP Stability Conjugate Reagent (ab171537) efficacy was verified by real-time stability studies that included independent testing of the enzyme activity and the immunological activity by ELISA.
The studies were conducted using a monoclonal IgG-HRP conjugate. Results of these analyses indicate superior performance of ab171537 compared to a common HRP conjugate diluent. The monoclonal IgG-HRP conjugate retained over 90% of its activity when stored in HRP Stability Conjugate Reagent for 24 months at room temperature.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab171537 尚未被引用在任何文献中。