Anti-H2R抗体(ab39964)
Key features and details
- Goat polyclonal to H2R
- Suitable for: Flow Cyt, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-H2R抗体
参阅全部 H2R 一抗 -
描述
山羊多克隆抗体to H2R -
宿主
Goat -
经测试应用
适用于: Flow Cyt, WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Dog -
免疫原
Synthetic peptide corresponding to Human H2R aa 335-349 (C terminal).
Sequence:QEEKPLKLQVWSGTE
-
阳性对照
- WB: Human tonsil tissue lysate; Flow Cyt: HeLa cells ICC/IF: HeLa cells
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading...
-
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab39964于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt |
Use a concentration of 10 µg/ml.
|
|
WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa.
A 1 hour primary incubation is recommended for this product. |
|
ICC/IF |
Use a concentration of 10 µg/ml.
|
说明 |
---|
Flow Cyt
Use a concentration of 10 µg/ml. |
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 40 kDa. A 1 hour primary incubation is recommended for this product. |
ICC/IF
Use a concentration of 10 µg/ml. |
靶标
-
功能
The H2 subclass of histamine receptors mediates gastric acid secretion. Also appears to regulate gastrointestinal motility and intestinal secretion. Possible role in regulating cell growth and differentiation. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and, through a separate G protein-dependent mechanism, the phosphoinositide/protein kinase (PKC) signaling pathway. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. -
细胞定位
Cell membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 3274 Human
- Entrez Gene: 15466 Mouse
- Omim: 142703 Human
- SwissProt: P25021 Human
- SwissProt: P97292 Mouse
- Unigene: 247885 Human
- Unigene: 57243 Mouse
-
别名
- Gastric receptor 1 antibody
- Gastric receptor I antibody
- H2R antibody
see all
图片
-
Anti-H2R antibody (ab39964) at 1 µg/ml + Human tonsil tissue lysates (35µg protein in RIPA buffer)
Predicted band size: 40 kDaDetected by chemiluminescence.
-
Immunocytochemistry/Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10µg/mL) followed by Alexa Fluor 488 secondary antibody (2µg/mL).
-
Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10µg/mL) followed by Alexa Fluor 488 secondary antibody (1µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
数据表及文件
-
Datasheet download
文献 (0)
ab39964 尚未被引用在任何文献中。