Anti-HPV11 E2抗体[10E12] (ab100968)
Key features and details
- Mouse monoclonal [10E12] to HPV11 E2
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Human papillomavirus
- Isotype: IgG1
概述
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产品名称
Anti-HPV11 E2抗体[10E12] -
描述
小鼠单克隆抗体[10E12] to HPV11 E2 -
宿主
Mouse -
特异性
Reacts in WB, and ELISA with HPV11 native E2 protein as well as recombinant E2 protein expressed in bacteria and Cos 7 cells. -
经测试应用
适用于: WB, ELISA, ICC/IFmore details
不适用于: IP -
种属反应性
与反应: Human papillomavirus -
免疫原
Recombinant full length HPV11 E2 protein purified from bacteria.
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表位
Mapped to amino acids 202-284 -
阳性对照
- U20S or Hela cells and lysates
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Store in the dark. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
纯化说明
Purified from bacteria by affinity chromatography. -
克隆
单克隆 -
克隆编号
10E12 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab100968于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/1000. Predicted molecular weight: 42 kDa.
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ELISA |
1/32000.
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ICC/IF |
Use at an assay dependent dilution.
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说明 |
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WB
1/1000. Predicted molecular weight: 42 kDa. |
ELISA
1/32000. |
ICC/IF
Use at an assay dependent dilution. |
靶标
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相关性
Human papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer. Human Papillomavirus (HPV) E2 proteins are the major viral regulators of transcription and replication during the viral life cycle. -
细胞定位
Host nucleus -
数据库链接
- SwissProt: P04015 Human papillomavirus
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别名
- Human papillomaviruses 11 E2 antibody
图片
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All lanes : Anti-HPV11 E2 antibody [10E12] (ab100968) at 1/1000 dilution
Lanes 1 & 4 : lysate of U20S cells transfected with plasmid expressing
HPV11 E2 protein
Lane 2 : lysate of plain U20S cells
Lane 3 : lysate of plain HeLa cells
Lane 5 : marker
Predicted band size: 42 kDa -
Immunofluorescence (IF) analysis of HPV11 E2 protein in U2OS cells.
IF was carried out with U2OS cell transfected with plasmid expressing HPV11 E2 protein. All the nuclei were stained by DAPI. Green color represents nucleus localized HPV11 E2 detected by ab100968. IF analysis of E2 protein shows similar result also in HeLa cells.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab100968 被引用在 2 文献中.
- Nanbo A et al. Epstein-Barr virus exploits host endocytic machinery for cell-to-cell viral transmission rather than a virological synapse. J Gen Virol 97:2989-3006 (2016). PubMed: 27655016
- Kivipõld P et al. DAXX modulates human papillomavirus early gene expression and genome replication in U2OS cells. Virol J 12:104 (2015). IF . PubMed: 26148509