Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (A549)
Permeabilization
Yes - Triton X, 0.025%
Specification
A549
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Methanol
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-T
Dilution
1/200
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Anti-rabbit IgG (Rockland)
Host species: Donkey
Clonality: Polyclonal
Conjugation: DyLight® 550
Host species: Donkey
Clonality: Polyclonal
Conjugation: DyLight® 550
Additional data
Additional Notes
This Ab works just fine with WB. But it does not work with ICC/IF.
Abcam response
Indeed, this antibody is not suitable for ICC/IF or Flow Cytometry, as tested by the lab.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Aug 10 2015