Application
Western blot
Sample
Mouse Cell lysate - whole cell (Raw 264.7)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
25 µg
Treatment
LPS 2 hours
Specification
Raw 264.7
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: BSA
Dilution
1/3000
Secondary antibody
Dilution
1/20000
Name
Non-Abcam antibody was used: anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
westpico
Negative control
Recombinant HP1g without kinase or ATP
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 25 kDa
Positive control
Recombinant HP1g with kinase and ATP
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Aug 07 2012