Application
Western blot
Sample
Human Cell lysate - nuclear (293T)
Gel Running Conditions
Reduced Denaturing (7.5%)
Loading amount
15 µg
Specification
293T
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Diluent: TBS-T Milk 1%
Dilution
1/100
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: anti Rb
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 21 kDa Non-specific: 37, 50 kDa
Additional data
Additional Notes
Although the are some nonspecific band (or dimers) we can conclude that antibody works properly.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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提交于 Dec 16 2013