Anti-HOXB13抗体[mAbcam53931] (ab53931)
Key features and details
- Mouse monoclonal [mAbcam53931] to HOXB13
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Human
- Isotype: IgG2a
Related conjugates and formulations
概述
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产品名称
Anti-HOXB13抗体[mAbcam53931]
参阅全部 HOXB13 一抗 -
描述
小鼠单克隆抗体[mAbcam53931] to HOXB13 -
宿主
Mouse -
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IFmore details -
种属反应性
与反应: Human
预测可用于: Mouse -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human HOXB13.
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阳性对照
- This antibody gave a positive signal against the HOXB13 (Human) Recombinant Protein.
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常规说明
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: 1% BSA -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
mAbcam53931 -
骨髓瘤
Sp2/0-Ag14 -
同种型
IgG2a -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53931于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt (Intra) |
Use 1µg for 106 cells.
ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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WB |
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 57 kDa corresponding to the tagged recombinant HOXB13 protein
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ICC/IF |
Use a concentration of 1 µg/ml.
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说明 |
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Flow Cyt (Intra)
Use 1µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
WB
Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 57 kDa corresponding to the tagged recombinant HOXB13 protein |
ICC/IF
Use a concentration of 1 µg/ml. |
靶标
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功能
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. -
序列相似性
Belongs to the Abd-B homeobox family.
Contains 1 homeobox DNA-binding domain. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 10481 Human
- Entrez Gene: 15408 Mouse
- Omim: 604607 Human
- SwissProt: Q92826 Human
- SwissProt: P70321 Mouse
- Unigene: 66731 Human
- Unigene: 57221 Mouse
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别名
- Homeo box B13 antibody
- Homeobox B13 antibody
- Homeobox protein Hox B13 antibody
see all
图片
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ab53931 staining HOXB13 in HepG2 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab53931 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue).
Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
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Anti-HOXB13 antibody [mAbcam53931] (ab53931) at 5 µg/ml + HOXB13 Recombinant protein at 0.1 µg
Secondary
Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 57 kDa why is the actual band size different from the predicted?
ab53931 recognizes the tagged HOXB13 recombinant protein which has an expected molecular weight of 57 kDa. -
Overlay histogram showing HeLa cells stained with ab53931 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53931, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
实验方案
数据表及文件
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Datasheet download
文献 (2)
ab53931 被引用在 2 文献中.
- Xu L et al. LncRNA SNHG14 regulates the DDP-resistance of non-small cell lung cancer cell through miR-133a/HOXB13 pathway. BMC Pulm Med 20:266 (2020). PubMed: 33059643
- Jiang Y et al. Repression of Hox genes by LMP1 in nasopharyngeal carcinoma and modulation of glycolytic pathway genes by HoxC8. Oncogene 34:6079-91 (2015). IHC-P ; Human . PubMed: 25745994