Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mouse lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization
Yes - 0.5% TritonX 100
Specification
Mouse lung
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking buffer
Dilution
1/1000
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Anti-rabbit IgG Fab2
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor 555
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor 555
Additional data
Additional Notes
The green signal in the image is Podoplanin (R&D Systems AF3244), another marker of Alveolar Type I cells.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Aug 11 2017