Application
ChIP
Detection step
Semiquantitative PCR
Sample
Human Cell lysate - whole cell (HEK293)
Specification
HEK293
Negative control
DW
No-antibody
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 8 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Duration of cross-linking step: 8 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Positive control
input (whole lysate)
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: go to Additional note #2
Additional data
Additional Notes
#1
I don't measure the chromatin concentration eveytime.
3ug HMGA antibody was used each samples.
#2
50mM Tris(pH8.0), 150mM NaCl, 1% TritonX-100, 0.1% Deoxycholate
with protease inhibitor.
Legend of qPCR
A,B negative control (DW)
C,D positive control (input)
E,F negative control (no-antibody: only beads)
G,H HMGA2 antibody
qPCR SYBR, StepOnePlus@ABI, N=2
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提交于 Sep 19 2014