Anti-HLA G抗体[MEM-G/1] (ab7759)
Key features and details
- Mouse monoclonal [MEM-G/1] to HLA G
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
概述
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产品名称
Anti-HLA G抗体[MEM-G/1]
参阅全部 HLA G 一抗 -
描述
小鼠单克隆抗体[MEM-G/1] to HLA G -
宿主
Mouse -
特异性
The antibody reacts with denaturated HLA-G heavy chain. -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Human -
免疫原
Recombinant full length denatured heavy chain (Human).
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阳性对照
- IHC-P: First trimester placenta and pulmonary diseases. WB: ab29745; HeLa and JEG-3 cell lysates.
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常规说明
This product was changed from ascites to tissue culture supernatant on 24th January 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
Purified from TCS. Purity >95% by SDS-PAGE. -
克隆
单克隆 -
克隆编号
MEM-G/1 -
同种型
IgG1 -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
应用 | Ab评论 | 说明 |
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IHC-P | (1) |
1/60 - 1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
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WB | (1) |
Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 38.2 kDa.
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说明 |
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IHC-P
1/60 - 1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol. |
WB
Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 38.2 kDa. |
靶标
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功能
Involved in the presentation of foreign antigens to the immune system. Plays a role in maternal tolerance of the fetus by mediating protection from the deleterious effects of natural killer cells, cytotoxic T lymphocytes, macrophages and mononuclear cells. -
组织特异性
Expressed in trophoblasts. -
序列相似性
Belongs to the MHC class I family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
细胞定位
Membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 3135 Human
- Omim: 142871 Human
- SwissProt: P17693 Human
- Unigene: 512152 Human
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别名
- B2 microglobulin antibody
- DADB-15K14.8 antibody
- HLA 6.0 antibody
see all
图片
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Immunohistochemistry staining with ab7759 of first trimester placenta (paraffin-embedded sections).
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All lanes : Anti-HLA G antibody [MEM-G/1] (ab7759) at 10 µg/ml
Lane 1 : Human placenta tissue lysate - total protein (ab29745)
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 38.2 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Additional bands at: 38 kDa. We are unsure as to the identity of these extra bands. -
The antibody ab7759 stains infiltrating macrophages in pulmonary diseases. In the top left corner see detail of macropage.
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All lanes : Anti-HLA G antibody [MEM-G/1] (ab7759) at 2 µg/ml
Lane 1 : JEG-3 cell line under reducing conditions.
Lane 2 : LNCap cell line under reducing conditions.
Lane 3 : JEG-3 cell line under non-reducing conditions.
Lane 4 : LNCap cell line under non-reducing conditions.
Secondary
All lanes : IRDye800-conjugated anti-mouse secondary antibody
Predicted band size: 38.2 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?Western blotting analysis of human HLA-G using ab7759 on lysates of JEG-3 cell line and LNCaP cell line (negative control) under reducing and non-reducing conditions.
Nitrocellulose membrane was probed with 2 µg/ml of ab7759 followed by IRDye800-conjugated anti-mouse secondary antibody.
数据表及文件
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SDS download
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Datasheet download
文献 (25)
ab7759 被引用在 25 文献中.
- Wang LJ et al. Functional antagonism between ΔNp63α and GCM1 regulates human trophoblast stemness and differentiation. Nat Commun 13:1626 (2022). PubMed: 35338152
- Bi Y et al. Cell fate roadmap of human primed-to-naive transition reveals preimplantation cell lineage signatures. Nat Commun 13:3147 (2022). PubMed: 35672317
- Tan JP et al. Establishment of human induced trophoblast stem cells via reprogramming of fibroblasts. Nat Protoc 17:2739-2759 (2022). PubMed: 36241724
- Qiu W et al. RNA Demethylase FTO Mediated RNA m6A Modification Is Involved in Maintaining Maternal-Fetal Interface in Spontaneous Abortion. Front Cell Dev Biol 9:617172 (2021). PubMed: 34350169
- Shahbazi MN et al. Developmental potential of aneuploid human embryos cultured beyond implantation. Nat Commun 11:3987 (2020). PubMed: 32778678