Anti-HLA E抗体[MEM-E/08] (ab11821)
Key features and details
- Mouse monoclonal [MEM-E/08] to HLA E
- Suitable for: IP, Flow Cyt, WB
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-HLA E抗体[MEM-E/08]
参阅全部 HLA E 一抗 -
描述
小鼠单克隆抗体[MEM-E/08] to HLA E -
宿主
Mouse -
特异性
The antibody recognized native surface-expressed HLA E, but not denaturated heavy chain of HLA E. The published results revealed that antibody is remarkably specific for HLA E, only with weak cross-reactivity with following classical MHC Class I molecules: HLA A24, B7, B27, B51, B54, C7. -
经测试应用
适用于: IP, Flow Cyt, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human HLA E.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
纯化说明
Purified from hybridoma culture supernatant by protein A-affinity chromatography. -
克隆
单克隆 -
克隆编号
MEM-E/08 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab11821于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IP |
Use at an assay dependent concentration.
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Flow Cyt | (1) |
Use at an assay dependent concentration.
Tested on HLA-E transfected human cell line LCL 721.221. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB |
Use at an assay dependent concentration.
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说明 |
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IP
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. Tested on HLA-E transfected human cell line LCL 721.221. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
Use at an assay dependent concentration. |
靶标
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相关性
HLA E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. -
细胞定位
Membrane; Single-pass type I membrane protein -
数据库链接
- Entrez Gene: 3133 Human
- Omim: 143010 Human
- SwissProt: P13747 Human
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别名
- HLA class I histocompatibility antigen E alpha chain antibody
- EA1.2 antibody
- EA2.1 antibody
see all
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab11821 被引用在 8 文献中.
- Arakawa A et al. ERAP1 Controls the Autoimmune Response against Melanocytes in Psoriasis by Generating the Melanocyte Autoantigen and Regulating Its Amount for HLA-C*06:02 Presentation. J Immunol 207:2235-2244 (2021). PubMed: 34580106
- Wang F et al. S6K-STING interaction regulates cytosolic DNA-mediated activation of the transcription factor IRF3. Nat Immunol 17:514-522 (2016). WB, IP ; Mouse . PubMed: 27043414
- Boura JS et al. Evaluation of gene delivery strategies to efficiently overexpress functional HLA-G on human bone marrow stromal cells. Mol Ther Methods Clin Dev 2014:N/A (2014). Flow Cyt ; Human . PubMed: 25279386
- Soland MA et al. Modulation of Human Mesenchymal Stem Cell Immunogenicity through Forced Expression of Human Cytomegalovirus US Proteins. PLoS One 7:e36163 (2012). Flow Cyt ; Human . PubMed: 22666319
- Heidenreich S et al. Impact of the NK cell receptor LIR-1 (ILT-2/CD85j/LILRB1) on cytotoxicity against multiple myeloma. Clin Dev Immunol 2012:652130 (2012). Flow Cyt ; Human . PubMed: 22844324
- Kalkunte SS et al. Vascular endothelial growth factor C facilitates immune tolerance and endovascular activity of human uterine NK cells at the maternal-fetal interface. J Immunol 182:4085-92 (2009). Flow Cyt ; Human . PubMed: 19299706
- Tennakoon DK et al. Therapeutic induction of regulatory, cytotoxic CD8+ T cells in multiple sclerosis. J Immunol 176:7119-29 (2006). Flow Cyt ; Human . PubMed: 16709875
- Brooks CR et al. The inhibitory receptor NKG2A determines lysis of vaccinia virus-infected autologous targets by NK cells. J Immunol 176:1141-7 (2006). Flow Cyt ; Human . PubMed: 16434388