Application
Western blot
Sample
Human Tissue lysate - whole (human colon lysate)
Loading amount
50 µg
Specification
human colon lysate
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 21°C
Other product details
Concentration
1 µg/ml
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 2%BSA in TBST
Secondary antibody
Name
Non-Abcam antibody was used: DAKO anti mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/3000
Detection
Detection method
ECL plus
Exposure
5 second(s)
Bands
Specific: 33 kDa Non-specific: 45 kDa
Positive control
this sample
Negative control
.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Feb 09 2012