Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Colorectal)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6 20 minutes
Permeabilization
No
Specification
Colorectal
Blocking step
hydrogen peroxide as blocking agent for 5 minute(s) · Concentration: 10%
Fixative
Formaldehyde
Other product details
Incubation time
15 minute(s)
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Polymer refine detection kit Leica
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
Nice crisp staining
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Feb 16 2016