HLA-DP overexpression 293T裂解物(whole cell) (ab94190)
概述
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产品名称
HLA-DP overexpression 293T裂解物(whole cell) -
常规说明
ab94190 is a 293T cell transfected lysate in which Human HLA DP has been transiently over-expressed using a pCMV-HLS DP plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view the preparation notes.
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经测试应用
适用于: WBmore details
性能
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Mycoplasma free
Yes -
形式
Liquid -
存放说明
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
存储溶液
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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研究领域
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背景
HLA-DPA1 belongs to the HLA class II alpha chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta (DPB) chain, both anchored in the membrane. It plays a vital role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (B lymphocytes, dendritic cells and macrophages). Within the DP molecule the alpha and beta chain contain the polymorphisms governing the peptide binding specificities, resulting in up to 4 different molecules.
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab94190于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent dilution.
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说明 |
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WB
Use at an assay dependent dilution. |
图片
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ab94190 at 15 μg/lane on an SDS-PAGE gel
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All lanes : Anti-HLA-DP antibody (ab88089) at 1/500 dilution
Lane 1 :HLA-DP overexpression 293T lysate (whole cell) (ab94190)
Lane 2 : 293T non-transfected Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab94190 尚未被引用在任何文献中。