生物素Anti-HLA Class I抗体[W6/32] (ab110665)
Key features and details
- Biotin Mouse monoclonal [W6/32] to HLA Class I
- Suitable for: Flow Cyt
- Reacts with: Cow, Cat, Human, Non human primates
- Conjugation: Biotin
- Isotype: IgG2a
Related conjugates and formulations
概述
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产品名称
生物素Anti-HLA Class I抗体[W6/32]
参阅全部 HLA Class I 一抗 -
描述
生物素小鼠单克隆抗体[W6/32] to HLA Class I -
宿主
Mouse -
偶联物
Biotin -
经测试应用
适用于: Flow Cytmore details -
种属反应性
与反应: Cow, Cat, Human, Non human primates
不与反应: Rabbit -
免疫原
Tissue, cells or virus corresponding to Human HLA Class I. Membrane of human tonsil cells
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常规说明
Suitable as a positive control for HLA tissue typing and crossmatching.
The purified antibody (>95% by SDS-PAGE) is conjugated with Biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Size exclusion -
Primary antibody说明
Suitable as a positive control for HLA tissue typing and crossmatching. -
克隆
单克隆 -
克隆编号
W6/32 -
同种型
IgG2a -
研究领域
相关产品
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Alternative Versions
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab110665于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Flow Cyt |
Use a concentration of 1 - 12 µg/ml.
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说明 |
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Flow Cyt
Use a concentration of 1 - 12 µg/ml. |
靶标
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相关性
HLA CLass I is involved in the presentation of foreign antigens to the immune system. -
细胞定位
Plasma membrane -
数据库链接
- Entrez Gene: 3105 Human
- Entrez Gene: 3133 Human
- Entrez Gene: 3134 Human
- Entrez Gene: 3135 Human
- Omim: 142800 Human
- SwissProt: O15506 Human
- SwissProt: P04439 Human
- SwissProt: P13747 Human
see all -
别名
- DKFZp686P19218 antibody
- EA1.2 antibody
- EA2.1 antibody
see all
图片
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Flow cytometry surface staining pattern of human peripheral whole blood stained using ab110665 at 4 μg/ml.
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Separation of human leukocytes (red-filled) from HLA Class I negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood using ab110665 at 4 μg/ml.
数据表及文件
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SDS download
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Datasheet download
文献 (4)
ab110665 被引用在 4 文献中.
- Wang D et al. Targeted Disruption of the ß2-Microglobulin Gene Minimizes the Immunogenicity of Human Embryonic Stem Cells. Stem Cells Transl Med 4:1234-45 (2015). PubMed: 26285657
- Choudhuri K et al. Peptide-major histocompatibility complex dimensions control proximal kinase-phosphatase balance during T cell activation. J Biol Chem 284:26096-105 (2009). PubMed: 19628870
- Tran TM et al. The epitope recognized by pan-HLA class I-reactive monoclonal antibody W6/32 and its relationship to unusual stability of the HLA-B27/beta2-microglobulin complex. Immunogenetics 53:440-6 (2001). PubMed: 11685454
- Kievits F & Ivanyi P Monomorphic anti-HLA monoclonal antibody (W6/32) recognizes polymorphic H-2 heavy-chain determinants exposed by association with bovine or human but not murine beta 2-microglobulin. Hum Immunol 20:115-26 (1987). PubMed: 3316135