Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (gel 15%)
Sample
Human Cell lysate - whole cell (astrocyte U251)
Specification
astrocyte U251
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: PBST
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Rabbit IRDye 800
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye 800CW
Detection
Detection method
Infrared Imaging System LI-COR
Exposure
10 minute(s) and 0 second(s)
Additional data
Additional Notes
I have the same problem with ab43014,lot # GR87684-3.it did not work too, There is no bands in WB.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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提交于 Oct 15 2013