Anti-HIV1 tat抗体[1] (ab42359)
Key features and details
- Mouse monoclonal [1] to HIV1 tat
- Suitable for: Radioimmunoprecipitation, IHC-P, ELISA, WB
- Reacts with: Human immunodeficiency virus
- Isotype: IgG1
概述
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产品名称
Anti-HIV1 tat抗体[1]
参阅全部 HIV1 tat 一抗 -
描述
小鼠单克隆抗体[1] to HIV1 tat -
宿主
Mouse -
特异性
This antibody reacts with the rTat consensus sequences of HIV1 subtypes A (DPVDPNLE), B (EPVDPRLE) and C (EPVDPNLE). -
经测试应用
适用于: Radioimmunoprecipitation, IHC-P, ELISA, WBmore details -
种属反应性
与反应: Human immunodeficiency virus -
免疫原
Recombinant fragment corresponding to HIV-1 HIV1 tat.
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表位
Mapped to amino acids 2-9 (EPVDPRLE - B subtype consensus). -
常规说明
Also known as clone 02-001.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
单克隆 -
克隆编号
1 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab42359于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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Radioimmunoprecipitation |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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ELISA |
Use a concentration of 0.02 - 0.05 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml.
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说明 |
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Radioimmunoprecipitation
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
ELISA
Use a concentration of 0.02 - 0.05 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. |
靶标
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相关性
HIV1 tat (Transactivator of Transcription) protein is a pleiotropic factor that induces a broad range of biological effects in numerous cell types. At the HIV promoter, tat is a powerful transactivator of gene expression which acts by inducing chromatin remodeling and by recruiting elongation-competent transcriptional complexes on to the viral LTR. -
细胞定位
Nuclear. Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region. -
数据库链接
- Entrez Gene: 155871 Human immunodeficiency virus
- SwissProt: P04608 Human immunodeficiency virus
- SwissProt: P04610 Human immunodeficiency virus
- SwissProt: Q6UFJ9 Human immunodeficiency virus
- SwissProt: Q900K4 Human immunodeficiency virus
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别名
- p14 antibody
- Protein Tat antibody
- Transactivating regulatory protein antibody
图片
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab42359 被引用在 11 文献中.
- Lai M et al. CRISPR/Cas9 Ablation of Integrated HIV-1 Accumulates Proviral DNA Circles with Reformed Long Terminal Repeats. J Virol 95:e0135821 (2021). PubMed: 34549986
- Pereira LA et al. MYB elongation is regulated by the nucleic acid binding of NF?B p50 to the intronic stem-loop region. PLoS One 10:e0122919 (2015). PubMed: 25853889
- Vandergaast R et al. Generation of West Nile virus infectious clones containing amino acid insertions between capsid and capsid anchor. Viruses 6:1637-53 (2014). WB . PubMed: 24721788
- Lai MC et al. Human DDX3 interacts with the HIV-1 Tat protein to facilitate viral mRNA translation. PLoS One 8:e68665 (2013). IP . PubMed: 23840900
- Wright CA et al. Effects of Tat proteins and Tat mutants of different human immunodeficiency virus type 1 clades on glial JC virus early and late gene transcription. J Gen Virol 94:514-23 (2013). PubMed: 23152365