Anti-FIST抗体(ab72538)
Key features and details
- Rabbit polyclonal to FIST
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
-
产品名称
Anti-FIST抗体 -
描述
兔多克隆抗体to FIST -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human FIST. NM_005734
Database link: Q9H422-1 -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.4
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
-
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab72538于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
WB |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 134 kDa (predicted molecular weight: 134 kDa).
|
|
IHC-P |
Use a concentration of 10 µg/ml.
|
说明 |
---|
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 134 kDa (predicted molecular weight: 134 kDa). |
IHC-P
Use a concentration of 10 µg/ml. |
靶标
-
功能
Seems to negatively regulate apoptosis by promoting FADD phosphorylation. Enhances androgen receptor-mediated transcription. May act as a transcriptional corepressor for NK homeodomain transcription factors. -
组织特异性
Overexpressed in multidrug resistant cells. Highly expressed in heart and skeletal muscle, and at lower levels in placenta, pancreas, brain, spleen, prostate, thymus, testis, small intestine, colon and leukocytes. Not found in liver and lung. -
序列相似性
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. HIPK subfamily.
Contains 1 protein kinase domain. -
翻译后修饰
Autophosphorylated, but autophosphorylation is not required for catalytic activity.
May be sumoylated. -
细胞定位
Cytoplasm. Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 10114 Human
- Entrez Gene: 15259 Mouse
- Entrez Gene: 83617 Rat
- Omim: 604424 Human
- SwissProt: Q9H422 Human
- SwissProt: Q9ERH7 Mouse
- SwissProt: O88850 Rat
- Unigene: 201918 Human
see all -
别名
- Androgen receptor interacting nuclear protein kinase antibody
- Androgen receptor-interacting nuclear protein kinase antibody
- ANPK antibody
see all
图片
-
ab72538 at 10µg/ml staining FIST in human testis by Immunohistochemistry using paraffin-embedded tissue.
-
All lanes : Anti-FIST antibody (ab72538) at 1 µg/ml
Lane 1 : Mouse testis lysate
Lane 2 : Mouse testis lysate with immunising peptide
Lane 3 : Rat testis lysate
Predicted band size: 134 kDa
Observed band size: 134 kDa
Additional bands at: 100 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
数据表及文件
-
Datasheet download
文献 (2)
ab72538 被引用在 2 文献中.
- Zhang H et al. ROR1 promotes the proliferation of endometrial cancer cells. Int J Clin Exp Pathol 10:10603-10610 (2017). PubMed: 31966402
- Huh JH et al. Dysregulation of miR-106a and miR-591 confers paclitaxel resistance to ovarian cancer. Br J Cancer 109:452-61 (2013). PubMed: 23807165