Application
Western blot
Sample
Human Cell lysate - whole cell (U2OS)
Loading amount
20 µg
Specification
U2OS
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
Other product details
Dilution
1/2000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk, 0.1% Tween-20, PBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-mouse HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: ~90 kDa Non-specific: ~50 (Faint) kDa
Additional data
Additional Notes
A good antibody.
A single species close to the predicted weight (87 kDa) was observed following SDS-PAGE.
A single species close to the predicted weight (87 kDa) was observed following SDS-PAGE.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jun 15 2007