Application
Western blot
Sample
Human Cell lysate - whole cell (HuH7 cells)
Gel Running Conditions
Reduced Denaturing (gel 8%)
Loading amount
25 µg
Treatment
Hypoxia 16h
Specification
HuH7 cells
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TTBS Milk
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Anti.mouse Igg,HRP linked
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Negative control
Cells in Normoxia
Exposure
6 minute(s) and 0 second(s)
Bands
Specific: 120 kDa
Positive control
Cells in hypoxia
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MISS. Virginia Gutiérrez
Verified customer
提交于 May 12 2016