I had no problems using this kit, it worked good on extracted lipids from liver tissue and values comparable to plasma levels.
Main limitation is the small amount of cholesterol assay buffer provided which just about fitted 100 samples. But we were aware of this and planned accordingly.
We extracted lipids from liver using one of the FAQ questions on the Abcam website related to ab65390 and that seemed to work.
All in all the experiment went good, we opted to assay total cholesterol and HDL only in plasma and liver samples which were 6 months old.
Main limitation is the small amount of cholesterol assay buffer provided which just about fitted 100 samples. But we were aware of this and planned accordingly.
We extracted lipids from liver using one of the FAQ questions on the Abcam website related to ab65390 and that seemed to work.
All in all the experiment went good, we opted to assay total cholesterol and HDL only in plasma and liver samples which were 6 months old.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jul 09 2015