概述

  • 产品名称
  • 描述
    兔多克隆抗体to HDAC7
  • 特异性
    The antibody detects ~105 kDa histone deacetylase 7. It does not cross-react with other HDAC proteins.
  • 经测试应用
    适用于: IHC-Fr, WB, IPmore details
  • 种属反应性
    与反应: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide:

    GILAEDRPSERLVE

    (Mouse)

性能

应用

Our Abpromise guarantee covers the use of ab1441 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-Fr Use a concentration of 30 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 102.9 kDa.
IP Use a concentration of 20 - 40 µg/ml.

靶标

  • 功能
    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer factors such as MEF2A, MEF2B and MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors (By similarity). May be involved in Epstein-Barr virus (EBV) latency, possibly by repressing the viral BZLF1 gene.
  • 序列相似性
    Belongs to the histone deacetylase family. HD type 2 subfamily.
  • 结构域
    The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
  • 翻译后修饰
    May be phosphorylated by CaMK1. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
  • 细胞定位
    Nucleus. Cytoplasm. In the nucleus, it associates with distinct subnuclear dot-like structures. Shuttles between the nucleus and the cytoplasm. Treatment with EDN1 results in shuttling from the nucleus to the perinuclear region. The export to cytoplasm depends on the interaction with the 14-3-3 protein YWHAE and may be due to its phosphorylation.
  • Information by UniProt
  • 数据库链接
  • 别名
    • DKFZP586J0917 antibody
    • FLJ99588 antibody
    • HD 7a antibody
    • HD7 antibody
    • HD7a antibody
    • HDAC 7 antibody
    • HDAC 7A antibody
    • Hdac7 antibody
    • HDAC7_HUMAN antibody
    • HDAC7A antibody
    • Histone deacetylase 7 antibody
    • Histone deacetylase 7A antibody
    • OTTHUMP00000202813 antibody
    • OTTHUMP00000202814 antibody
    see all

文献

This product has been referenced in:
  • Zhou B  et al. Splicing of histone deacetylase 7 modulates smooth muscle cell proliferation and neointima formation through nuclear ß-catenin translocation. Arterioscler Thromb Vasc Biol 31:2676-84 (2011). Read more (PubMed: 21836063) »
  • Chang S  et al. Histone deacetylase 7 maintains vascular integrity by repressing matrix metalloproteinase 10. Cell 126:321-34 (2006). ChIP ; Human . Read more (PubMed: 16873063) »

See all 2 Publications for this product

客户评价及客户问答

Application
Flow Cytometry
Fixation
Paraformaldehyde
Permeabilization
Yes - BD permeabilization buffer
Sample
Mouse Cell (Mouse thymus tissue)
Specification
Mouse thymus tissue
Gating Strategy
CD45 positives
Preparation
Cell harvesting/tissue preparation method: Digestion with Collagenase D
Sample buffer: PBS 2% FCS
Username

Mr. Sanjay Gawade

Verified customer

提交于 Jan 29 2015

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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