HDAC6 overexpression 293T裂解物(whole cell) (ab94173)
概述
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产品名称
HDAC6 overexpression 293T裂解物(whole cell)
参阅全部 HDAC6 细胞裂解液 -
常规说明
ab94173 is a 293T cell transfected lysate in which Human HDAC6 has been transiently over-expressed using a pCMV-HDAC6 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed about how the transfected lysate was prepared view preparation notes
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经测试应用
适用于: WBmore details
性能
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Mycoplasma free
Yes -
形式
Liquid -
存放说明
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
存储溶液
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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研究领域
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背景
Function: Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes (By similarity). Plays a central role in microtubule-dependent cell motility via deacetylation of tubulin. Similarity: Belongs to the histone deacetylase family. HD type 2 subfamily. Contains 1 UBP-type zinc finger. PTM: Ubiquitinated. Its polyubiquitination however does not lead to its degradation. Sumoylated in vitro.
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab94173于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use at an assay dependent dilution.
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说明 |
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WB
Use at an assay dependent dilution. |
图片
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ab94173 at 15µg/lane on an SDS-PAGE gel.
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All lanes : Anti-HDAC6 antibody (ab56926) at 1/500 dilution
Lane 1 :HDAC6 overexpression 293T lysate (whole cell) (ab94173)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab94173 尚未被引用在任何文献中。