Application
Western blot
Sample
Mouse Cell lysate - nuclear (Lung)
Loading amount
10 µg
Specification
Lung
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA PBS-Tween (0.1%)
Secondary antibody
Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG (H+L chain specific)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Dilution
1/1000
Detection
Detection method
ECF
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 55kDa kDa Non-specific: light one at ~35kDa kDa
Positive control
Whole Cell THP-1 Extract
Additional data
Additional Notes
Used Optitran BA-S 85 Nitrocellulose
Lane 1. Whole Cell THP-1 extract
Lane 2. Nuclear Mouse Lung extract
Lane 1. Whole Cell THP-1 extract
Lane 2. Nuclear Mouse Lung extract
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Aug 23 2007