Application
Western blot
Sample
Monkey Cell lysate - whole cell (Brain)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
12 µg
Specification
Brain
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Diluent: TBS+ 1% Milk + .1% Tween)
Dilution
1/5000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Cell Signaling 7074S Donkey anti-rabbit-HRP
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
50 second(s)
Bands
Specific: ~60 kDa
Positive control
Brain lysate samples were prepared from non-human primate (macaque) gray matter (cortex). Total protein concentration was measured by BCA assay.
Additional data
Additional Notes
Lane 1: 25 ug NHP Brain lysate
Lane 2: 12.5 ug NHP Brain lysate
Lane 3: 6.25 ug NHP Brain lysate
Lane 4: 3.3 ug NHP Brain lysate
Lane 5: 1.6 ug NHP Brain lysate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 May 29 2015