Application
Western blot
Sample
Human Cell lysate - whole cell (lymphoblastoid cell line)
Loading amount
100000 cells
Specification
lymphoblastoid cell line
Treatment
shRNA
Gel Running Conditions
Non-reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/10000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 5% milk
Secondary antibody
Name
Non-Abcam antibody was used: cell signalling
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
20 second(s)
Bands
Specific: 55 kDa
Additional data
Additional Notes
1 scrambled shRNA
2 shRNA against HDAC2
2 shRNA against HDAC2
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Mar 15 2010