Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (HL-1 cardiac cell line)
Specification
HL-1 cardiac cell line
Fixative
Methanol
Blocking step
Goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Other product details
Dilution
1/100
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 4% goat serum in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti Mouse CY3
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/250
Additional data
Additional Notes
The antibody does give some unexpected nuclear staining.
Abcam response
Thank you for your abreview. Abcam are unsure about the staining pattern of HCN1 in cardiac cells and would appreciate feedback from other customers using this antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Nov 15 2010