Application
Western blot
Sample
Rat Cell lysate - whole cell (whole bladder)
Gel Running Conditions
Reduced Denaturing (Tris-HCl 10%)
Loading amount
60 µg
Specification
whole bladder
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TBS-T w/ 5% (v/v) milk
Concentration
1 µg/ml
Secondary antibody
Dilution
1/25000
Name
Non-Abcam antibody was used: goat anti-rabbit IgG-HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
our samples
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 20-30 kDa Non-specific: 55, 40 kDa
Positive control
our samples
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 May 04 2011