Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Mouse Tissue lysate - whole (Heart)
Specification
Heart
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA/TBST
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: Goat-anti-mouse IgG
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Bands
Specific: ~50 and ~80 kDa Non-specific: ~25 kDa
Exposure
10 second(s)
Negative control
no
Positive control
no
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. Arcan Guven
Verified customer
提交于 Sep 03 2014