Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (brain)
Permeabilization
No
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Paraformaldehyde
Other product details
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: 1% n-serum in PBS
Dilution
1/100
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Goat-anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
Detection system: ABC(peroxidase) 1:500 in 1% n-Serum in PBS 90 min 20°C
the peroxidase was detected by DAB-Ni.
30µm thick frozen sections.
Free floating technique.
Important! Don't use any kind of detergent!
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ute Neubacher
Verified customer
提交于 Feb 17 2009