Application
Western blot
Sample
Mouse Cell lysate - whole cell (Colonic mucosa)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Treatment
6 hours following 12Gy gamma radiation
Specification
Colonic mucosa
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/50
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Dako anti-rabbit Igg
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 74 kDa
Additional data
Additional Notes
Image: Lanes 1 and 2 are colonic mucosal lysates from untreated mice. Lanes 3 and 4 are colonic mucosal lysates from 12Gy gamma-irradiated mice culled 6 hours post-irradiation. Mw band is at 74kDa.
Abcam response
Asked user to specify the templerature of incubation with the primary antibody;
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 May 22 2009