Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Small intestine)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM citric acid buffer
Permeabilization
No
Specification
Small intestine
Blocking step
Serum as blocking agent for 45 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Formalin
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 10% serum
Dilution
1/1000
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: anti-rabbit Igg
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 May 22 2009