Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma)
Loading amount
30 µg
Specification
Glioblastoma
Gel Running Conditions
Reduced Denaturing (4-15%)
Blocking step
Pierce TBS Super block Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Dilution
1/800
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Pierce TBS Super block Blocking Buffer
Secondary antibody
Name
Non-Abcam antibody was used: IRDye 680RD Goat anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 680
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 680
Dilution
1/5000
Detection
Detection method
Odyssey
Exposure
15 minute(s) and 0 second(s)
Bands
Specific: 71 and 110 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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提交于 May 02 2013